Detailed information of TA system TA106782

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2384..2810
• Replicon: plasmid p51008369SK1_A
• Accession: NZ_CP029974
• Organism: Escherichia coli strain 51008369SK1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DNQ41_RS24845
• Protein ID: WP_001312861.1
• Coordinates: 2384..2542 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	2586..2810 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNQ41_RS24820	241..1062	-	822	WP_001234445.1	DUF945 domain-containing protein	-
DNQ41_RS24825	1173..1469	-	297	WP_001272251.1	hypothetical protein	-
DNQ41_RS27905	1769..2146	+	378	Protein_2	hypothetical protein	-
DNQ41_RS24845	2384..2542	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2586..2810	-	225	NuclAT_0	-	Antitoxin
-	2586..2810	-	225	NuclAT_0	-	Antitoxin
-	2586..2810	-	225	NuclAT_0	-	Antitoxin
-	2586..2810	-	225	NuclAT_0	-	Antitoxin
DNQ41_RS27745	2622..2801	+	180	WP_001309233.1	hypothetical protein	-
DNQ41_RS24850	2822..3541	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
DNQ41_RS24855	3538..3972	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DNQ41_RS24860	4041..6064	-	2024	Protein_7	ParB/RepB/Spo0J family partition protein	-
DNQ41_RS24865	6125..6358	-	234	WP_000006003.1	DUF905 family protein	-
DNQ41_RS24870	6416..6943	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2 / erm(B)	-	1..71718	71718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T106782 WP_001312861.1 NZ_CP029974:c2542-2384 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T106782 NZ_CP029974:c2542-2384 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT106782 NZ_CP029974:c2810-2586 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin