Detailed information of TA system TA106600

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1479743..1480571
• Replicon: chromosome
• Accession: NZ_CP029741
• Organism: Escherichia coli strain AR_0085

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AM464_RS07665
• Protein ID: WP_089643420.1
• Coordinates: 1479743..1480120 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2Y8PC11
• Locus tag: AM464_RS07670
• Protein ID: WP_001696847.1
• Coordinates: 1480203..1480571 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM464_RS07635	1475382..1477223	+	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-
AM464_RS07640	1477302..1477808	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
AM464_RS07650	1478119..1478964	-	846	WP_001696810.1	DUF4942 domain-containing protein	-
AM464_RS07655	1479049..1479246	-	198	WP_001696812.1	DUF957 domain-containing protein	-
AM464_RS07660	1479258..1479746	-	489	WP_000761678.1	hypothetical protein	-
AM464_RS07665	1479743..1480120	-	378	WP_089643420.1	TA system toxin CbtA family protein	Toxin
AM464_RS07670	1480203..1480571	-	369	WP_001696847.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM464_RS07675	1480734..1480955	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM464_RS07680	1481018..1481494	-	477	WP_001186747.1	RadC family protein	-
AM464_RS07685	1481510..1481983	-	474	WP_001696846.1	antirestriction protein	-
AM464_RS07690	1482077..1482322	-	246	WP_000680586.1	hypothetical protein	-
AM464_RS07695	1482322..1483140	-	819	WP_001696845.1	DUF945 domain-containing protein	-
AM464_RS07710	1483480..1484550	-	1071	WP_097743645.1	patatin-like phospholipase family protein	-
AM464_RS07715	1484547..1485452	-	906	WP_110844019.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.13 Da
• Isoelectric Point: 9.2315

>T106600 WP_089643420.1 NZ_CP029741:c1480120-1479743 [Escherichia coli]
MKTLPDTHVRKASRCPSPVTVWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T106600 NZ_CP029741:c1480120-1479743 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGAAGGCATCGCGCTGCCCGTCTCCCGTCACCGTCTGGCAGACACTACTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13751.47 Da
• Isoelectric Point: 5.9618

>AT106600 WP_001696847.1 NZ_CP029741:c1480571-1480203 [Escherichia coli]
VSDTLHETNYPDDNNDRSWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT106600 NZ_CP029741:c1480571-1480203 [Escherichia coli]
GTGTCAGACACACTCCATGAAACAAATTATCCCGACGACAACAACGACCGCTCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Y8PC11