Detailed information of TA system TA106520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1393252..1393849
• Replicon: chromosome
• Accession: NZ_CP029722
• Organism: Klebsiella pneumoniae strain AR_0140

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: AM387_RS07015
• Protein ID: WP_004893639.1
• Coordinates: 1393532..1393849 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: AM387_RS07010
• Protein ID: WP_004142561.1
• Coordinates: 1393252..1393539 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM387_RS06980	1389329..1389577	+	249	WP_032410665.1	DUF1158 domain-containing protein	-
AM387_RS06985	1389595..1389936	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
AM387_RS06990	1389967..1391082	-	1116	Protein_1338	MBL fold metallo-hydrolase	-
AM387_RS06995	1391262..1391846	+	585	WP_110561233.1	TetR/AcrR family transcriptional regulator	-
AM387_RS07000	1391846..1392214	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
AM387_RS07005	1392334..1392987	+	654	WP_068815271.1	oxygen-insensitive NAD(P)H nitroreductase	-
AM387_RS07010	1393252..1393539	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
AM387_RS07015	1393532..1393849	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM387_RS07020	1394034..1395077	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
AM387_RS07030	1395743..1396609	-	867	WP_068815272.1	helix-turn-helix transcriptional regulator	-
AM387_RS07035	1396718..1398145	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T106520 WP_004893639.1 NZ_CP029722:c1393849-1393532 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T106520 NZ_CP029722:c1393849-1393532 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT106520 WP_004142561.1 NZ_CP029722:c1393539-1393252 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT106520 NZ_CP029722:c1393539-1393252 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1