Detailed information of TA system TA106500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4625274..4625927
• Replicon: chromosome
• Accession: NZ_CP029715
• Organism: Serratia marcescens strain AR_0131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM378_RS21905
• Protein ID: WP_033645671.1
• Coordinates: 4625274..4625624 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: AM378_RS21910
• Protein ID: WP_019453270.1
• Coordinates: 4625628..4625927 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM378_RS21875	4620536..4621225	-	690	WP_033648575.1	trehalose-6-phosphate synthase	-
AM378_RS21880	4621239..4621649	-	411	WP_033648574.1	GNAT family N-acetyltransferase	-
AM378_RS21885	4621679..4623049	-	1371	WP_033648572.1	glutamine synthetase family protein	-
AM378_RS21890	4623295..4623612	+	318	WP_041037744.1	hypothetical protein	-
AM378_RS21895	4623603..4623998	-	396	WP_033648570.1	hypothetical protein	-
AM378_RS21900	4623998..4625002	-	1005	WP_043148198.1	hypothetical protein	-
AM378_RS21905	4625274..4625624	+	351	WP_033645671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM378_RS21910	4625628..4625927	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
AM378_RS21915	4625917..4626834	-	918	WP_033648568.1	LysR family transcriptional regulator	-
AM378_RS21920	4627003..4627701	+	699	WP_033648567.1	CoA transferase subunit A	-
AM378_RS21925	4627713..4628366	+	654	WP_033648566.1	CoA transferase subunit B	-
AM378_RS21930	4628380..4629564	+	1185	WP_033648565.1	acetyl-CoA C-acetyltransferase	-
AM378_RS21935	4629583..4630506	+	924	WP_033648563.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T106500 WP_033645671.1 NZ_CP029715:4625274-4625624 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T106500 NZ_CP029715:4625274-4625624 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAGCAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCCGGGCCTTCTCTCCGGCGGCCTTTTGTCGACGTGCTGAAAGGTTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCCTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATTGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT106500 WP_019453270.1 NZ_CP029715:4625628-4625927 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT106500 NZ_CP029715:4625628-4625927 [Serratia marcescens]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAACGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3