Detailed information of TA system TA105952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 995150..995963
• Replicon: chromosome
• Accession: NZ_CP029590
• Organism: Klebsiella pneumoniae strain DA33144

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: DLJ67_RS05005
• Protein ID: WP_101516303.1
• Coordinates: 995150..995686 (-)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: DLJ67_RS05010
• Protein ID: WP_023343051.1
• Coordinates: 995697..995963 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ67_RS05000	993981..995102	+	1122	WP_012737335.1	cupin domain-containing protein	-
DLJ67_RS05005	995150..995686	-	537	WP_101516303.1	GNAT family N-acetyltransferase	Toxin
DLJ67_RS05010	995697..995963	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
DLJ67_RS05015	996066..997499	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
DLJ67_RS05020	997489..998172	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
DLJ67_RS05025	998345..999730	+	1386	WP_040182471.1	efflux transporter outer membrane subunit	-
DLJ67_RS05030	999748..1000092	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19951.87 Da
• Isoelectric Point: 5.7068

>T105952 WP_101516303.1 NZ_CP029590:c995686-995150 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSN
LLFYPTKSIEQLFTDDES

Nucleotide

• Length: 537 bp

>T105952 NZ_CP029590:c995686-995150 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGT
TTGTTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAAC
TTACTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT105952 WP_023343051.1 NZ_CP029590:c995963-995697 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT105952 NZ_CP029590:c995963-995697 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
GGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA