Detailed information of TA system TA105929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3098..3623
• Replicon: plasmid pDA33140-112
• Accession: NZ_CP029583
• Organism: Klebsiella pneumoniae strain DA33140

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: DLJ83_RS27045
• Protein ID: WP_012539978.1
• Coordinates: 3098..3403 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: DLJ83_RS27050
• Protein ID: WP_015632547.1
• Coordinates: 3405..3623 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ83_RS29465	374..487	+	114	WP_032072094.1	hypothetical protein	-
DLJ83_RS27030	615..872	+	258	WP_032433931.1	hypothetical protein	-
DLJ83_RS27035	930..1709	-	780	WP_015632549.1	site-specific integrase	-
DLJ83_RS27040	1907..2911	-	1005	WP_029497484.1	hypothetical protein	-
DLJ83_RS27045	3098..3403	-	306	WP_012539978.1	type II toxin-antitoxin system toxin CcdB	Toxin
DLJ83_RS27050	3405..3623	-	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
DLJ83_RS27060	4223..5497	+	1275	WP_015632546.1	hypothetical protein	-
DLJ83_RS27065	5570..5755	-	186	WP_032433930.1	hypothetical protein	-
DLJ83_RS27070	6333..6683	+	351	WP_015632544.1	hypothetical protein	-
DLJ83_RS27075	6876..8378	-	1503	WP_048296350.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..112141	112141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.27 Da
• Isoelectric Point: 6.4661

>T105929 WP_012539978.1 NZ_CP029583:c3403-3098 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T105929 NZ_CP029583:c3403-3098 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT105929 WP_015632547.1 NZ_CP029583:c3623-3405 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT105929 NZ_CP029583:c3623-3405 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86