Detailed information of TA system TA105902

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111882..112121
• Replicon: plasmid pDA33137-178
• Accession: NZ_CP029580
• Organism: Escherichia coli strain DA33137

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: DLJ64_RS27985
• Protein ID: WP_023144756.1
• Coordinates: 111882..112016 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	112061..112121 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ64_RS27950	107893..108294	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
DLJ64_RS28780	108227..108484	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
DLJ64_RS27955	108577..109230	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
DLJ64_RS28975	109328..109468	-	141	WP_001333237.1	hypothetical protein	-
DLJ64_RS27965	110170..111027	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
DLJ64_RS27970	111020..111094	-	75	WP_031943482.1	RepA leader peptide Tap	-
DLJ64_RS28980	111091..111225	-	135	Protein_130	protein CopA/IncA	-
DLJ64_RS27980	111331..111585	-	255	WP_000083850.1	replication regulatory protein RepA	-
DLJ64_RS27985	111882..112016	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	112061..112121	+	61	NuclAT_1	-	Antitoxin
-	112061..112121	+	61	NuclAT_1	-	Antitoxin
-	112061..112121	+	61	NuclAT_1	-	Antitoxin
-	112061..112121	+	61	NuclAT_1	-	Antitoxin
DLJ64_RS27990	112088..112374	-	287	Protein_133	DUF2726 domain-containing protein	-
DLJ64_RS28000	112887..113099	-	213	WP_013023861.1	hypothetical protein	-
DLJ64_RS28005	113230..113790	-	561	WP_000139341.1	fertility inhibition protein FinO	-
DLJ64_RS28010	113845..114591	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / blaCTX-M-14 / aadA5 / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / cmlA1	senB	1..178078	178078
	-	inside	Integron	-	senB	27..178076	178049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T105902 WP_023144756.1 NZ_CP029580:c112016-111882 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T105902 NZ_CP029580:c112016-111882 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT105902 NZ_CP029580:112061-112121 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin