Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3532109..3532734 Replicon chromosome
Accession NZ_CP029388
Organism Klebsiella pneumoniae subsp. pneumoniae strain SCKP040074

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CVG30_RS19590 Protein ID WP_002882817.1
Coordinates 3532351..3532734 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CVG30_RS19585 Protein ID WP_004150355.1
Coordinates 3532109..3532351 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVG30_RS19560 3528100..3528699 + 600 WP_004151865.1 glucose-1-phosphatase -
CVG30_RS19565 3528693..3529553 + 861 WP_004146232.1 virulence factor BrkB family protein -
CVG30_RS19570 3529550..3529987 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
CVG30_RS19575 3530032..3530973 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CVG30_RS19580 3530987..3531904 - 918 WP_004178029.1 alpha/beta hydrolase -
CVG30_RS19585 3532109..3532351 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
CVG30_RS19590 3532351..3532734 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CVG30_RS19595 3532908..3533837 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CVG30_RS19600 3533834..3534469 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CVG30_RS19605 3534466..3535368 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T105589 WP_002882817.1 NZ_CP029388:3532351-3532734 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T105589 NZ_CP029388:3532351-3532734 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT105589 WP_004150355.1 NZ_CP029388:3532109-3532351 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT105589 NZ_CP029388:3532109-3532351 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References