Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4002855..4003509 Replicon chromosome
Accession NZ_CP029328
Organism Escherichia coli strain A1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY52_RS19355 Protein ID WP_000244772.1
Coordinates 4002855..4003262 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY52_RS19360 Protein ID WP_000354046.1
Coordinates 4003243..4003509 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY52_RS19335 (3998812) 3998812..4000545 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
AOY52_RS19340 (4000551) 4000551..4001261 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY52_RS19345 (4001286) 4001286..4002182 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY52_RS19350 (4002294) 4002294..4002815 + 522 WP_001055874.1 flavodoxin FldB -
AOY52_RS19355 (4002855) 4002855..4003262 - 408 WP_000244772.1 protein YgfX Toxin
AOY52_RS19360 (4003243) 4003243..4003509 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY52_RS19365 (4003752) 4003752..4004732 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY52_RS19370 (4005104) 4005104..4006084 + 981 WP_000399679.1 IS110-like element IS621 family transposase -
AOY52_RS19375 (4006207) 4006207..4006866 - 660 WP_000250274.1 hemolysin III family protein -
AOY52_RS19380 (4007030) 4007030..4007341 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4005104..4006084 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T105456 WP_000244772.1 NZ_CP029328:c4003262-4002855 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T105456 NZ_CP029328:c4003262-4002855 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT105456 WP_000354046.1 NZ_CP029328:c4003509-4003243 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT105456 NZ_CP029328:c4003509-4003243 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References