Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3939721..3940375 Replicon chromosome
Accession NZ_CP029327
Organism Escherichia coli strain A37

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY90_RS18960 Protein ID WP_000244781.1
Coordinates 3939721..3940128 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY90_RS18965 Protein ID WP_000354046.1
Coordinates 3940109..3940375 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY90_RS18940 (3935678) 3935678..3937411 - 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -
AOY90_RS18945 (3937417) 3937417..3938127 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY90_RS18950 (3938152) 3938152..3939048 - 897 WP_097751990.1 site-specific tyrosine recombinase XerD -
AOY90_RS18955 (3939160) 3939160..3939681 + 522 WP_001055874.1 flavodoxin FldB -
AOY90_RS18960 (3939721) 3939721..3940128 - 408 WP_000244781.1 protein YgfX Toxin
AOY90_RS18965 (3940109) 3940109..3940375 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY90_RS18970 (3940618) 3940618..3941598 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
AOY90_RS18975 (3941675) 3941675..3942334 - 660 WP_000250274.1 hemolysin III family protein -
AOY90_RS18980 (3942498) 3942498..3942809 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AOY90_RS18985 (3942854) 3942854..3944287 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T105432 WP_000244781.1 NZ_CP029327:c3940128-3939721 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T105432 NZ_CP029327:c3940128-3939721 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGACGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT105432 WP_000354046.1 NZ_CP029327:c3940375-3940109 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT105432 NZ_CP029327:c3940375-3940109 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References