Detailed information of TA system TA105412

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1018398..1019205
• Replicon: chromosome
• Accession: NZ_CP029327
• Organism: Escherichia coli strain A37

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E2S9W0
• Locus tag: AOY90_RS04725
• Protein ID: WP_042004473.1
• Coordinates: 1018398..1018784 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: AOY90_RS04730
• Protein ID: WP_001285482.1
• Coordinates: 1018831..1019205 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY90_RS04700 (1013765)	1013765..1015358	+	1594	Protein_898	IS66 family transposase	-
AOY90_RS04705 (1015506)	1015506..1016494	+	989	Protein_899	IS630 family transposase	-
AOY90_RS04710 (1016573)	1016573..1016981	-	409	Protein_900	DUF5983 family protein	-
AOY90_RS04720 (1018315)	1018315..1018401	-	87	Protein_902	DUF5983 family protein	-
AOY90_RS04725 (1018398)	1018398..1018784	-	387	WP_042004473.1	TA system toxin CbtA family protein	Toxin
AOY90_RS04730 (1018831)	1018831..1019205	-	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY90_RS04735 (1019255)	1019255..1019899	-	645	WP_000086759.1	hypothetical protein	-
AOY90_RS04740 (1019918)	1019918..1020139	-	222	WP_097752265.1	DUF987 domain-containing protein	-
AOY90_RS04745 (1020202)	1020202..1020678	-	477	WP_001313574.1	RadC family protein	-
AOY90_RS04750 (1020694)	1020694..1021167	-	474	WP_001313575.1	antirestriction protein	-
AOY90_RS04755 (1021261)	1021261..1021506	-	246	WP_001164966.1	hypothetical protein	-
AOY90_RS04760 (1021506)	1021506..1022327	-	822	WP_097752264.1	DUF932 domain-containing protein	-
AOY90_RS04765 (1022427)	1022427..1022660	-	234	WP_001213776.1	DUF905 family protein	-
AOY90_RS04770 (1022778)	1022778..1023230	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-115)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14595.66 Da
• Isoelectric Point: 8.2918

>T105412 WP_042004473.1 NZ_CP029327:c1018784-1018398 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 387 bp

>T105412 NZ_CP029327:c1018784-1018398 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCCCGCCGGGCAACCGGCCTGAT
GACCCGCAGCAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT105412 WP_001285482.1 NZ_CP029327:c1019205-1018831 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT105412 NZ_CP029327:c1019205-1018831 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0P7R0L9