Detailed information of TA system TA105393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 35127..35794
• Replicon: plasmid unnamedc
• Accession: NZ_CP029283
• Organism: Xanthomonas phaseoli pv. phaseoli strain Px100

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3T0G501
• Locus tag: DGM85_RS22435
• Protein ID: WP_022557732.1
• Coordinates: 35127..35543 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DGM85_RS22440
• Protein ID: WP_215825407.1
• Coordinates: 35540..35794 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DGM85_RS22840	30671..33175	+	2505	WP_236493581.1	type III secretion system effector protein XopC	-
DGM85_RS22425 (DGM85_23615)	33728..34480	-	753	Protein_40	IS3 family transposase	-
DGM85_RS22430 (DGM85_23620)	34559..35113	-	555	WP_033482006.1	plasmid pRiA4b ORF-3 family protein	-
DGM85_RS22435 (DGM85_23625)	35127..35543	-	417	WP_022557732.1	type II toxin-antitoxin system VapC family toxin	Toxin
DGM85_RS22440 (DGM85_23630)	35540..35794	-	255	WP_215825407.1	plasmid stabilization protein	Antitoxin
DGM85_RS22445 (DGM85_23635)	36006..36599	+	594	WP_022560487.1	recombinase family protein	-
DGM85_RS22450 (DGM85_23640)	36596..39625	+	3030	WP_022560486.1	Tn3 family transposase	-
DGM85_RS22455 (DGM85_23645)	39657..39997	-	341	Protein_46	transposase	-
DGM85_RS22460 (DGM85_23655)	40249..40569	+	321	WP_033481941.1	ParC family partition-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50192	50192
	-	inside	IScluster/Tn	-	-	27008..45466	18458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14833.04 Da
• Isoelectric Point: 4.6812

>T105393 WP_022557732.1 NZ_CP029283:c35543-35127 [Xanthomonas phaseoli pv. phaseoli]
MIVLDTNVVSEAMKPEPHPSVRTWLNDQAAETLYLSSVTLAELLFGIAALPAGKRKDMLEQALDGLMGLFRDRVLPFDID
AARRYAELAVTAKIGGRGFPTPDGYIAAIAASRGFIVASRDTAPYEAAGVTVINPWEG

Nucleotide

• Length: 417 bp

>T105393 NZ_CP029283:c35543-35127 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9402.83 Da
• Isoelectric Point: 8.9326

>AT105393 WP_215825407.1 NZ_CP029283:c35794-35540 [Xanthomonas phaseoli pv. phaseoli]
MAMLTVRNLPDDVHRALRMRAAQNGHSTEAEVREILATAVKPETRVRLGEALAALGRKIGLTNEDFEVFDRVRDKTPAIP
PRLE

Nucleotide

• Length: 255 bp

>AT105393 NZ_CP029283:c35794-35540 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCATCCCG
CCGAGGCTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A3T0G501