Detailed information of TA system TA105312

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 49340..50141
• Replicon: chromosome
• Accession: NZ_CP029242
• Organism: Escherichia coli strain ECCRA-119

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: C7235_RS00285
• Protein ID: WP_001094437.1
• Coordinates: 49340..49717 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2S1XZS0
• Locus tag: C7235_RS00290
• Protein ID: WP_072645736.1
• Coordinates: 49764..50141 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7235_RS00250	44698..45621	-	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
C7235_RS00255	45732..46916	-	1185	WP_001172876.1	sugar efflux transporter	-
C7235_RS00260	47313..47474	-	162	Protein_46	virulence RhuM family protein	-
C7235_RS00270	47709..48553	-	845	Protein_47	DUF4942 domain-containing protein	-
C7235_RS00275	48638..48835	-	198	WP_000772032.1	DUF957 domain-containing protein	-
C7235_RS00280	48855..49343	-	489	WP_000761715.1	hypothetical protein	-
C7235_RS00285	49340..49717	-	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
C7235_RS00290	49764..50141	-	378	WP_072645736.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C7235_RS00295	50304..50525	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C7235_RS00300	50588..51064	-	477	WP_001186775.1	RadC family protein	-
C7235_RS00305	51080..51553	-	474	WP_000855064.1	antirestriction protein	-
C7235_RS00315	51895..52713	-	819	WP_072645737.1	DUF945 domain-containing protein	-
C7235_RS00325	52868..53026	-	159	WP_001323397.1	DUF905 family protein	-
C7235_RS00330	53097..54059	-	963	Protein_57	autotransporter adhesin family protein	-
C7235_RS00335	54109..54813	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T105312 WP_001094437.1 NZ_CP029242:c49717-49340 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T105312 NZ_CP029242:c49717-49340 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13787.61 Da
• Isoelectric Point: 5.9541

>AT105312 WP_072645736.1 NZ_CP029242:c50141-49764 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT105312 NZ_CP029242:c50141-49764 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A2S1XZS0