Detailed information of TA system TA105064

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1778978..1779192
• Replicon: chromosome
• Accession: NZ_CP029154
• Organism: Clostridioides difficile strain CD161

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: DDG63_RS21580
• Protein ID: WP_003429855.1
• Coordinates: 1778978..1779139 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1779059..1779192 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDG63_RS08525	1774318..1774551	+	234	WP_003433536.1	hypothetical protein	-
DDG63_RS08530	1774561..1774962	+	402	WP_009888836.1	hypothetical protein	-
DDG63_RS08535	1774956..1775303	+	348	WP_003433540.1	hypothetical protein	-
DDG63_RS08540	1775303..1775728	+	426	WP_003433542.1	HK97 gp10 family phage protein	-
DDG63_RS08545	1775721..1776158	+	438	WP_003433544.1	hypothetical protein	-
DDG63_RS21575	1776151..1776327	+	177	WP_003433548.1	hypothetical protein	-
DDG63_RS08555	1776328..1777638	+	1311	WP_003433550.1	phage tail sheath family protein	-
DDG63_RS08560	1777655..1778125	+	471	WP_003429851.1	phage tail tube protein	-
DDG63_RS08565	1778197..1778637	+	441	WP_003429853.1	phage portal protein	-
DDG63_RS21580	1778978..1779139	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1779059..1779192	-	134	NuclAT_2	-	Antitoxin
-	1779059..1779192	-	134	NuclAT_3	-	Antitoxin
-	1779537..1779585	-	49	NuclAT_7	-	-
-	1779537..1779585	-	49	NuclAT_8	-	-
DDG63_RS08580	1780464..1781039	+	576	WP_043897361.1	SHOCT domain-containing protein	-
DDG63_RS08585	1781343..1781516	+	174	WP_003433557.1	hypothetical protein	-
DDG63_RS21410	1781640..1782467	+	828	WP_003433559.1	hypothetical protein	-
DDG63_RS21585	1782516..1782692	+	177	WP_003433561.1	hypothetical protein	-
DDG63_RS08595	1783123..1783683	+	561	WP_021387709.1	ribosomal protein L7/L12	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T105064 WP_003429855.1 NZ_CP029154:1778978-1779139 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T105064 NZ_CP029154:1778978-1779139 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT105064 NZ_CP029154:c1779192-1779059 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin