Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 64108..64751 Replicon plasmid unnamed1
Accession NZ_CP029135
Organism Klebsiella pneumoniae strain AR376

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CSC13_RS00465 Protein ID WP_001044770.1
Coordinates 64335..64751 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CSC13_RS00460 Protein ID WP_001261282.1
Coordinates 64108..64338 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC13_RS28725 59872..59976 + 105 WP_032409716.1 hypothetical protein -
CSC13_RS00415 60105..60362 + 258 WP_000764642.1 hypothetical protein -
CSC13_RS00420 60420..61196 - 777 WP_000015958.1 site-specific integrase -
CSC13_RS00425 61193..61936 - 744 WP_000129823.1 hypothetical protein -
CSC13_RS00430 61987..62337 - 351 WP_000493378.1 hypothetical protein -
CSC13_RS00445 63145..63597 + 453 WP_004201034.1 hypothetical protein -
CSC13_RS00455 63924..64151 - 228 Protein_72 hypothetical protein -
CSC13_RS00460 64108..64338 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CSC13_RS00465 64335..64751 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CSC13_RS00470 64825..65316 + 492 Protein_75 AAA family ATPase -
CSC13_RS00475 65373..66070 + 698 Protein_76 IS1 family transposase -
CSC13_RS00480 66084..66212 - 129 Protein_77 IS110 family transposase -
CSC13_RS00485 66494..66673 - 180 Protein_78 IS3 family transposase -
CSC13_RS00490 66754..68262 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
CSC13_RS00500 68935..69622 - 688 Protein_80 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..70613 70613


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T105025 WP_001044770.1 NZ_CP029135:64335-64751 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T105025 NZ_CP029135:64335-64751 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT105025 WP_001261282.1 NZ_CP029135:64108-64338 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT105025 NZ_CP029135:64108-64338 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References