Detailed information of TA system TA104589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 104386..104971
• Replicon: plasmid pAbW39-2
• Accession: NZ_CP029007
• Organism: Acinetobacter pittii strain AbW39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: DDE02_RS21315
• Protein ID: WP_000897311.1
• Coordinates: 104651..104971 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DDE02_RS21310
• Protein ID: WP_000369784.1
• Coordinates: 104386..104658 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDE02_RS21280 (DDE02_21575)	99807..100604	+	798	WP_004757464.1	phosphonate ABC transporter, permease protein PhnE	-
DDE02_RS21285 (DDE02_21580)	100646..101653	+	1008	WP_005071472.1	phosphonate dehydrogenase	-
DDE02_RS21290 (DDE02_21585)	101650..102507	+	858	WP_000609033.1	LysR family transcriptional regulator	-
DDE02_RS21295 (DDE02_21590)	102833..103045	-	213	WP_001123161.1	hypothetical protein	-
DDE02_RS21300 (DDE02_21595)	103161..103462	-	302	Protein_121	IS3 family transposase	-
DDE02_RS21305 (DDE02_21600)	103553..104353	-	801	WP_000604996.1	DUF2971 domain-containing protein	-
DDE02_RS21310 (DDE02_21605)	104386..104658	-	273	WP_000369784.1	NadS family protein	Antitoxin
DDE02_RS21315 (DDE02_21610)	104651..104971	-	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DDE02_RS21320 (DDE02_21615)	105650..106582	-	933	WP_252618458.1	IS5-like element IS17 family transposase	-
DDE02_RS21325 (DDE02_21620)	106636..107073	+	438	Protein_126	IS3 family transposase	-
DDE02_RS21330 (DDE02_21630)	107331..108563	+	1233	WP_001255535.1	ATP-binding protein	-
DDE02_RS21335 (DDE02_21640)	108835..109164	-	330	WP_000506885.1	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..149504	149504
	-	inside	IScluster/Tn	-	-	103127..107073	3946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T104589 WP_000897311.1 NZ_CP029007:c104971-104651 [Acinetobacter pittii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T104589 NZ_CP029007:c104971-104651 [Acinetobacter pittii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT104589 WP_000369784.1 NZ_CP029007:c104658-104386 [Acinetobacter pittii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT104589 NZ_CP029007:c104658-104386 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50