104575

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54306..55042 Replicon plasmid unnamed6
Accession NZ_CP028995
Organism Klebsiella pneumoniae strain AR_0079

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM458_RS28595 Protein ID WP_003026803.1
Coordinates 54560..55042 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM458_RS28590 Protein ID WP_003026799.1
Coordinates 54306..54572 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM458_RS28545 50368..50730 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM458_RS28550 50780..51130 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM458_RS28555 51488..51757 + 270 WP_004152102.1 hypothetical protein -
AM458_RS28560 51745..52320 + 576 WP_004152103.1 hypothetical protein -
AM458_RS28565 52351..52845 + 495 WP_004152104.1 DNA-binding protein -
AM458_RS28570 52889..53257 + 369 WP_004152105.1 hypothetical protein -
AM458_RS28575 53291..53494 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AM458_RS28580 53543..53800 + 258 WP_004152107.1 hypothetical protein -
AM458_RS28585 53876..54130 + 255 WP_004152108.1 hypothetical protein -
AM458_RS28590 54306..54572 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM458_RS28595 54560..55042 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM458_RS28600 55243..56646 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM458_RS28605 56675..57307 - 633 WP_001567369.1 hypothetical protein -
AM458_RS28610 57742..58746 - 1005 Protein_58 IS110 family transposase -
AM458_RS28615 58825..59382 - 558 WP_003100847.1 recombinase family protein -
AM458_RS28620 59376..59747 - 372 WP_003100853.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaDHA-1 / qnrB4 / blaTEM-1B - 1..152176 152176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T104575 WP_003026803.1 NZ_CP028995:54560-55042 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T104575 NZ_CP028995:54560-55042 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT104575 WP_003026799.1 NZ_CP028995:54306-54572 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT104575 NZ_CP028995:54306-54572 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References