Detailed information of TA system TA104463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1834341..1834994
• Replicon: chromosome
• Accession: NZ_CP028949
• Organism: Serratia marcescens strain AR_0121

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM368_RS08890
• Protein ID: WP_033645671.1
• Coordinates: 1834644..1834994 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: AM368_RS08885
• Protein ID: WP_019453270.1
• Coordinates: 1834341..1834640 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM368_RS08860	1829762..1830685	-	924	WP_033648563.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
AM368_RS08865	1830704..1831888	-	1185	WP_033648565.1	acetyl-CoA C-acetyltransferase	-
AM368_RS08870	1831902..1832555	-	654	WP_033648566.1	CoA transferase subunit B	-
AM368_RS08875	1832567..1833265	-	699	WP_033648567.1	CoA transferase subunit A	-
AM368_RS08880	1833434..1834351	+	918	WP_033648568.1	LysR family transcriptional regulator	-
AM368_RS08885	1834341..1834640	-	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
AM368_RS08890	1834644..1834994	-	351	WP_033645671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM368_RS08895	1835266..1836270	+	1005	WP_043148198.1	hypothetical protein	-
AM368_RS08900	1836270..1836665	+	396	WP_033648570.1	hypothetical protein	-
AM368_RS08905	1836656..1836973	-	318	WP_041037744.1	hypothetical protein	-
AM368_RS08910	1837219..1838589	+	1371	WP_033648572.1	glutamine synthetase family protein	-
AM368_RS08915	1838619..1839029	+	411	WP_033648574.1	GNAT family N-acetyltransferase	-
AM368_RS08920	1839043..1839732	+	690	WP_033648575.1	trehalose-6-phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T104463 WP_033645671.1 NZ_CP028949:c1834994-1834644 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T104463 NZ_CP028949:c1834994-1834644 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAGCAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCCGGGCCTTCTCTCCGGCGGCCTTTTGTCGACGTGCTGAAAGGTTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCCTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATTGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT104463 WP_019453270.1 NZ_CP028949:c1834640-1834341 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT104463 NZ_CP028949:c1834640-1834341 [Serratia marcescens]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAACGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3