Detailed information of TA system TA104356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 58934..59551
• Replicon: plasmid unnamed
• Accession: NZ_CP028898
• Organism: Dongshaea marina strain DM2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DB847_RS23990
• Protein ID: WP_108653146.1
• Coordinates: 58934..59116 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DB847_RS23995
• Protein ID: WP_108653147.1
• Coordinates: 59141..59551 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB847_RS23955	54072..54266	-	195	WP_108653139.1	hypothetical protein	-
DB847_RS23960	54376..54669	-	294	WP_108653140.1	hypothetical protein	-
DB847_RS23965	55120..55284	+	165	WP_108653141.1	DUF3950 domain-containing protein	-
DB847_RS23970	55407..55850	-	444	WP_108653142.1	hypothetical protein	-
DB847_RS23975	55843..56565	-	723	WP_108653143.1	AAA family ATPase	-
DB847_RS23985	58132..58722	+	591	WP_108653145.1	recombinase family protein	-
DB847_RS23990	58934..59116	+	183	WP_108653146.1	type II toxin-antitoxin system HicA family toxin	Toxin
DB847_RS23995	59141..59551	+	411	WP_108653147.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DB847_RS24000	59723..59965	+	243	WP_108653148.1	hypothetical protein	-
DB847_RS24005	60374..60973	+	600	WP_108653149.1	hypothetical protein	-
DB847_RS24010	61024..61608	+	585	WP_159084843.1	helix-turn-helix domain-containing protein	-
DB847_RS24015	61630..62052	+	423	WP_108653151.1	thioesterase family protein	-
DB847_RS24020	62177..62635	-	459	WP_108653152.1	helix-turn-helix domain-containing protein	-
DB847_RS24030	64126..64530	+	405	WP_108653154.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..79320	79320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6893.10 Da
• Isoelectric Point: 10.6914

>T104356 WP_108653146.1 NZ_CP028898:58934-59116 [Dongshaea marina]
VNSREFIRMIEDDGWYLVKIKGSHHHYKHPTKPGKVTVGHPKKDFPPKTLATMLKQAGLK

Nucleotide

• Length: 183 bp

>T104356 NZ_CP028898:58934-59116 [Dongshaea marina]
GTGAATAGCCGAGAATTTATCAGGATGATTGAGGATGACGGGTGGTACTTAGTTAAGATTAAAGGCAGTCATCATCACTA
CAAGCATCCAACTAAGCCAGGCAAGGTTACGGTAGGGCACCCAAAGAAAGACTTTCCACCCAAAACCTTAGCAACCATGT
TAAAACAAGCTGGGCTCAAGTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15126.06 Da
• Isoelectric Point: 4.2461

>AT104356 WP_108653147.1 NZ_CP028898:59141-59551 [Dongshaea marina]
MLYTIAIEPGDEQHAYGVIVPDIPGCFSGGDTLDEAYKNAKEAIELHLEGLAEDRDKLPEAQPIDIYANDPEYQGFIMGV
IEIDIAPYLGKSKKINVTLPEYVIKQIDRAAETSPSYRDRSNFLLVASLHELSQTK

Nucleotide

• Length: 411 bp

>AT104356 NZ_CP028898:59141-59551 [Dongshaea marina]
ATGCTTTATACAATCGCAATCGAGCCTGGAGATGAACAGCACGCCTATGGGGTGATTGTTCCTGATATTCCCGGCTGCTT
CTCCGGAGGCGATACTCTCGACGAAGCATACAAAAATGCCAAAGAGGCGATAGAATTACACCTCGAAGGGCTGGCAGAGG
ATCGAGATAAACTGCCTGAGGCTCAGCCGATCGATATCTATGCGAACGACCCTGAGTACCAGGGCTTTATTATGGGCGTG
ATAGAGATTGATATAGCCCCATACCTGGGGAAAAGTAAGAAGATCAATGTCACTTTGCCTGAGTATGTTATCAAGCAGAT
CGACAGAGCGGCAGAAACCAGCCCCTCATATCGAGACCGCTCTAATTTTCTGCTTGTTGCATCATTGCACGAGCTATCCC
AAACAAAGTAG