Detailed information of TA system TA104355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 3061998..3062764
• Replicon: chromosome
• Accession: NZ_CP028897
• Organism: Dongshaea marina strain DM2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: DB847_RS14495
• Protein ID: WP_108652985.1
• Coordinates: 3062357..3062764 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: DB847_RS14490
• Protein ID: WP_108651339.1
• Coordinates: 3061998..3062273 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB847_RS14460	3057800..3058309	-	510	WP_108651334.1	BREX-3 system P-loop-containing protein BrxF	-
DB847_RS14465	3058312..3059250	-	939	WP_199911577.1	WYL domain-containing protein	-
DB847_RS14470	3059405..3059926	-	522	WP_108651335.1	DUF4236 domain-containing protein	-
DB847_RS14475	3060020..3060430	-	411	WP_108651336.1	hypothetical protein	-
DB847_RS14480	3060467..3060886	-	420	WP_159084640.1	hypothetical protein	-
DB847_RS14485	3061016..3061639	-	624	WP_108651338.1	hypothetical protein	-
DB847_RS14490	3061998..3062273	+	276	WP_108651339.1	DUF1778 domain-containing protein	Antitoxin
DB847_RS14495	3062357..3062764	+	408	WP_108652985.1	GNAT family N-acetyltransferase	Toxin
DB847_RS14500	3062768..3063373	-	606	WP_108651340.1	tyrosine-type recombinase/integrase	-
DB847_RS14505	3063882..3065212	+	1331	Protein_2835	HipA domain-containing protein	-
DB847_RS14510	3065209..3065517	+	309	WP_108651341.1	helix-turn-helix domain-containing protein	-
DB847_RS14515	3066072..3067496	-	1425	WP_108650532.1	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3036953..3070095	33142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (8-113)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15068.22 Da
• Isoelectric Point: 7.2006

>T104355 WP_108652985.1 NZ_CP028897:3062357-3062764 [Dongshaea marina]
MASQQAVKDNSRTFVIEDPQREEWIIGYYSLTMIPVDLGVLPEKLQKKHQSARSGGLIARLAVDRRYTGNGYGEWLLIDS
LHKLLQASESVGFPVVVVDAKEGAADFYRKFGFQSFADTPNKLFMTIADIRASLG

Nucleotide

• Length: 408 bp

>T104355 NZ_CP028897:3062357-3062764 [Dongshaea marina]
ATGGCCAGCCAGCAAGCGGTGAAAGACAATAGCAGAACCTTTGTAATTGAAGATCCTCAAAGAGAGGAGTGGATCATTGG
TTACTATTCATTAACCATGATCCCTGTCGACCTGGGCGTTCTTCCGGAAAAATTACAAAAAAAGCATCAGAGTGCCCGCT
CAGGAGGGCTCATCGCCCGGCTGGCCGTCGATCGTCGCTACACCGGTAATGGTTATGGTGAGTGGCTACTCATCGACAGT
CTGCATAAGTTGCTTCAGGCAAGCGAATCAGTTGGCTTTCCTGTGGTCGTTGTCGATGCGAAAGAGGGCGCTGCAGATTT
TTACAGAAAGTTTGGCTTCCAATCCTTTGCGGATACCCCCAACAAACTGTTTATGACCATTGCCGATATCCGAGCCAGCC
TAGGCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10091.56 Da
• Isoelectric Point: 9.9229

>AT104355 WP_108651339.1 NZ_CP028897:3061998-3062273 [Dongshaea marina]
MANIRPRITARVDVQTQELLIRASALAGMSNVNSFVLNAAVEKAKKIVEQEQMLQLSEQDALLLAKALDTPARPNSRLQQ
AAQRYQESKDS

Nucleotide

• Length: 276 bp

>AT104355 NZ_CP028897:3061998-3062273 [Dongshaea marina]
ATGGCAAATATTCGGCCTCGCATTACGGCGCGAGTAGACGTTCAGACTCAGGAACTGCTTATCAGAGCATCAGCTCTTGC
CGGGATGAGTAATGTAAACTCTTTTGTACTCAATGCAGCGGTTGAGAAAGCAAAGAAAATTGTTGAGCAAGAGCAGATGT
TGCAGCTTTCAGAGCAAGATGCGCTGCTGCTCGCTAAAGCTCTGGATACGCCAGCCCGCCCTAACTCCAGGTTACAACAG
GCAGCACAACGATATCAGGAGAGCAAAGACTCGTGA