Detailed information of TA system TA104244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4645168..4645787
• Replicon: chromosome
• Accession: NZ_CP028826
• Organism: Pseudomonas fluorescens strain MS82

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: DBV33_RS21095
• Protein ID: WP_199912984.1
• Coordinates: 4645168..4645464 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0C1WFF8
• Locus tag: DBV33_RS21100
• Protein ID: WP_039773303.1
• Coordinates: 4645467..4645787 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBV33_RS21065	4640523..4641719	-	1197	WP_108563022.1	hypothetical protein	-
DBV33_RS21070	4641806..4642525	-	720	WP_108563023.1	hypothetical protein	-
DBV33_RS21075	4642556..4642918	-	363	WP_108563024.1	hypothetical protein	-
DBV33_RS21080	4642963..4643778	-	816	WP_108563025.1	sulfite exporter TauE/SafE family protein	-
DBV33_RS21085	4643891..4644658	+	768	WP_108563026.1	helix-turn-helix transcriptional regulator	-
DBV33_RS21090	4644752..4645069	+	318	WP_085654862.1	hypothetical protein	-
DBV33_RS21095	4645168..4645464	+	297	WP_199912984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBV33_RS21100	4645467..4645787	+	321	WP_039773303.1	helix-turn-helix domain-containing protein	Antitoxin
DBV33_RS21105	4645836..4647353	-	1518	WP_108563027.1	amidase	-
DBV33_RS21110	4647466..4648539	-	1074	WP_108563028.1	MBL fold metallo-hydrolase	-
DBV33_RS21115	4648754..4649362	+	609	WP_064383649.1	TetR/AcrR family transcriptional regulator	-
DBV33_RS21120	4649509..4650561	+	1053	WP_108563029.1	diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10787.29 Da
• Isoelectric Point: 8.4926

>T104244 WP_199912984.1 NZ_CP028826:4645168-4645464 [Pseudomonas fluorescens]
MPTDVQDVFGYALDLAQSGLRHPNAKPLKGFGGAGVLELIEDFDTNTYRAVYTVRFGNAIYVLHCFQKKSTTGIKTAQCD
VDLIRKRLQAAQDHAKGT

Nucleotide

• Length: 297 bp

>T104244 NZ_CP028826:4645168-4645464 [Pseudomonas fluorescens]
ATGCCCACTGATGTACAGGATGTTTTTGGTTACGCACTGGATTTGGCCCAAAGCGGTCTCAGACATCCAAATGCAAAACC
TCTGAAGGGGTTTGGAGGCGCGGGTGTCCTCGAGTTGATTGAGGACTTTGATACGAATACCTACCGCGCCGTTTACACGG
TCCGTTTTGGCAATGCGATTTACGTCCTGCATTGTTTTCAGAAAAAGTCGACCACCGGTATAAAGACCGCGCAGTGCGAT
GTCGATCTGATCCGAAAGAGACTTCAAGCCGCTCAGGACCACGCGAAAGGAACTTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11690.60 Da
• Isoelectric Point: 8.0411

>AT104244 WP_039773303.1 NZ_CP028826:4645467-4645787 [Pseudomonas fluorescens]
MIEIEEGSGNVYQDLGLPDASEMQVKSQLAAKIGEIIKARHLTQSQASEILGLSQPKLSEMLRGKFRGISEAKMMECLSH
LGRDVQIVVKPAPRSRKEGRIEVVFA

Nucleotide

• Length: 321 bp

>AT104244 NZ_CP028826:4645467-4645787 [Pseudomonas fluorescens]
ATGATTGAAATCGAAGAAGGCAGCGGCAACGTCTATCAAGACCTCGGCTTGCCCGACGCCAGTGAAATGCAGGTCAAGTC
TCAACTCGCCGCAAAGATCGGTGAAATCATCAAAGCGCGTCATCTCACTCAATCTCAAGCTTCTGAAATCTTGGGGTTAT
CACAACCCAAGCTATCCGAAATGCTGAGAGGAAAATTCCGCGGCATCAGTGAGGCCAAGATGATGGAATGCCTTTCCCAC
CTCGGCCGCGATGTGCAGATTGTGGTCAAACCGGCCCCCCGCTCTCGCAAAGAGGGCCGAATTGAGGTGGTGTTCGCTTG
A

Structures

Antitoxin

• AlphaFold DB: A0A0C1WFF8