Detailed information of TA system TA104102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3705391..3706228
• Replicon: chromosome
• Accession: NZ_CP028772
• Organism: Escherichia coli strain A2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY53_RS18155
• Protein ID: WP_000227784.1
• Coordinates: 3705686..3706228 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY53_RS18150
• Protein ID: WP_001297137.1
• Coordinates: 3705391..3705702 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY53_RS18125 (3700411)	3700411..3701358	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY53_RS18130 (3701380)	3701380..3703371	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY53_RS18135 (3703361)	3703361..3703975	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY53_RS18140 (3703975)	3703975..3704304	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY53_RS18145 (3704316)	3704316..3705206	+	891	WP_000971336.1	heme o synthase	-
AOY53_RS18150 (3705391)	3705391..3705702	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY53_RS18155 (3705686)	3705686..3706228	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY53_RS18160 (3706284)	3706284..3707219	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY53_RS18165 (3707627)	3707627..3708991	+	1365	WP_001000978.1	MFS transporter	-
AOY53_RS18170 (3709119)	3709119..3709610	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY53_RS18175 (3709778)	3709778..3710689	+	912	WP_000705849.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T104102 WP_000227784.1 NZ_CP028772:3705686-3706228 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T104102 NZ_CP028772:3705686-3706228 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT104102 WP_001297137.1 NZ_CP028772:3705391-3705702 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT104102 NZ_CP028772:3705391-3705702 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3