Detailed information of TA system TA104007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4865271..4865873
• Replicon: chromosome
• Accession: NZ_CP028768
• Organism: Escherichia coli strain A6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY57_RS24010
• Protein ID: WP_000897302.1
• Coordinates: 4865562..4865873 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY57_RS24005
• Protein ID: WP_000356397.1
• Coordinates: 4865271..4865561 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY57_RS23980 (4861344)	4861344..4862246	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY57_RS23985 (4862243)	4862243..4862878	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY57_RS23990 (4862875)	4862875..4863804	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY57_RS23995 (4864020)	4864020..4864238	-	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY57_RS24000 (4864634)	4864634..4864912	-	279	WP_001296612.1	hypothetical protein	-
AOY57_RS24005 (4865271)	4865271..4865561	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY57_RS24010 (4865562)	4865562..4865873	-	312	WP_000897302.1	hypothetical protein	Toxin
AOY57_RS24015 (4866102)	4866102..4867010	+	909	WP_001331553.1	alpha/beta hydrolase	-
AOY57_RS24020 (4867074)	4867074..4868015	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY57_RS24025 (4868060)	4868060..4868497	-	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
AOY57_RS24030 (4868494)	4868494..4869366	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY57_RS24035 (4869360)	4869360..4869959	-	600	WP_001314324.1	glucose-1-phosphatase	-
AOY57_RS24040 (4870058)	4870058..4870843	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T104007 WP_000897302.1 NZ_CP028768:c4865873-4865562 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T104007 NZ_CP028768:c4865873-4865562 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT104007 WP_000356397.1 NZ_CP028768:c4865561-4865271 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT104007 NZ_CP028768:c4865561-4865271 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416