Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1017893..1018547 Replicon chromosome
Accession NZ_CP028768
Organism Escherichia coli strain A6

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY57_RS05040 Protein ID WP_000244781.1
Coordinates 1018140..1018547 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY57_RS05035 Protein ID WP_000354046.1
Coordinates 1017893..1018159 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY57_RS05015 (1013971) 1013971..1015404 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
AOY57_RS05020 (1015449) 1015449..1015760 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AOY57_RS05025 (1015924) 1015924..1016583 + 660 WP_000250274.1 hemolysin III family protein -
AOY57_RS05030 (1016660) 1016660..1017640 - 981 WP_021513792.1 tRNA-modifying protein YgfZ -
AOY57_RS05035 (1017893) 1017893..1018159 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY57_RS05040 (1018140) 1018140..1018547 + 408 WP_000244781.1 protein YgfX Toxin
AOY57_RS05045 (1018587) 1018587..1019108 - 522 WP_001055874.1 flavodoxin FldB -
AOY57_RS05050 (1019220) 1019220..1020116 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY57_RS05055 (1020141) 1020141..1020851 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY57_RS05060 (1020857) 1020857..1022590 + 1734 WP_042020700.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T103986 WP_000244781.1 NZ_CP028768:1018140-1018547 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103986 NZ_CP028768:1018140-1018547 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTAATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTACGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103986 WP_000354046.1 NZ_CP028768:1017893-1018159 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103986 NZ_CP028768:1017893-1018159 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References