Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4074016..4074670 Replicon chromosome
Accession NZ_CP028766
Organism Escherichia coli strain A8

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AOY61_RS19900 Protein ID WP_000244772.1
Coordinates 4074016..4074423 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY61_RS19905 Protein ID WP_000354046.1
Coordinates 4074404..4074670 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY61_RS19880 (4069973) 4069973..4071706 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
AOY61_RS19885 (4071712) 4071712..4072422 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY61_RS19890 (4072447) 4072447..4073343 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY61_RS19895 (4073455) 4073455..4073976 + 522 WP_001055874.1 flavodoxin FldB -
AOY61_RS19900 (4074016) 4074016..4074423 - 408 WP_000244772.1 protein YgfX Toxin
AOY61_RS19905 (4074404) 4074404..4074670 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY61_RS19910 (4074913) 4074913..4075893 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY61_RS19915 (4076089) 4076089..4076748 - 660 WP_000250274.1 hemolysin III family protein -
AOY61_RS19920 (4076912) 4076912..4077223 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AOY61_RS19925 (4077268) 4077268..4078701 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
AOY61_RS19930 (4078758) 4078758..4079501 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T103954 WP_000244772.1 NZ_CP028766:c4074423-4074016 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103954 NZ_CP028766:c4074423-4074016 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103954 WP_000354046.1 NZ_CP028766:c4074670-4074404 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103954 NZ_CP028766:c4074670-4074404 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References