Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 732670..733397 Replicon chromosome
Accession NZ_CP028764
Organism Escherichia coli strain A10

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag AOY63_RS03530 Protein ID WP_000550189.1
Coordinates 732670..732984 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag AOY63_RS03535 Protein ID WP_000560253.1
Coordinates 732981..733397 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY63_RS03510 (728836) 728836..729822 - 987 WP_001315023.1 Gfo/Idh/MocA family oxidoreductase -
AOY63_RS03515 (729901) 729901..730584 - 684 WP_001183059.1 vancomycin high temperature exclusion protein -
AOY63_RS03520 (730661) 730661..731164 - 504 WP_001305999.1 M48 family metallopeptidase -
AOY63_RS03525 (731249) 731249..732385 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AOY63_RS03530 (732670) 732670..732984 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
AOY63_RS03535 (732981) 732981..733397 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AOY63_RS03540 (733442) 733442..735460 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AOY63_RS03545 (735631) 735631..737982 - 2352 WP_000695464.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T103887 WP_000550189.1 NZ_CP028764:732670-732984 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T103887 NZ_CP028764:732670-732984 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT103887 WP_000560253.1 NZ_CP028764:732981-733397 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT103887 NZ_CP028764:732981-733397 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACGCCCAGGCGCTGGAACTGGTCGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References