Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1020047..1020588 Replicon chromosome
Accession NZ_CP028759
Organism Escherichia coli strain A15

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag AOY68_RS04680 Protein ID WP_000615982.1
Coordinates 1020047..1020325 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY68_RS04685 Protein ID WP_000729704.1
Coordinates 1020328..1020588 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY68_RS04665 (1017629) 1017629..1018207 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY68_RS04670 (1018413) 1018413..1019180 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY68_RS04675 (1019151) 1019151..1019891 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY68_RS04680 (1020047) 1020047..1020325 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY68_RS04685 (1020328) 1020328..1020588 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY68_RS04690 (1020774) 1020774..1021547 + 774 WP_000543899.1 C40 family peptidase -
AOY68_RS04695 (1021604) 1021604..1021960 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY68_RS04700 (1021953) 1021953..1022231 - 279 WP_042099148.1 type II toxin-antitoxin system HicA family toxin -
AOY68_RS04705 (1022336) 1022336..1024048 - 1713 Protein_902 flagellar biosynthesis protein FlhA -
AOY68_RS04710 (1024020) 1024020..1024805 + 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / rhs/PAAR / gmhA/lpcA 1002283..1022231 19948


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T103760 WP_000615982.1 NZ_CP028759:c1020325-1020047 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103760 NZ_CP028759:c1020325-1020047 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103760 WP_000729704.1 NZ_CP028759:c1020588-1020328 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103760 NZ_CP028759:c1020588-1020328 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References