Detailed information of TA system TA103758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 153534..154136
• Replicon: chromosome
• Accession: NZ_CP028759
• Organism: Escherichia coli strain A15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY68_RS00750
• Protein ID: WP_000897305.1
• Coordinates: 153534..153845 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY68_RS00755
• Protein ID: WP_000356397.1
• Coordinates: 153846..154136 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY68_RS00720 (148564)	148564..149349	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY68_RS00725 (149448)	149448..150047	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY68_RS00730 (150041)	150041..150913	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY68_RS00735 (150910)	150910..151347	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY68_RS00740 (151392)	151392..152333	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
AOY68_RS00745 (152397)	152397..153305	-	909	WP_001299484.1	alpha/beta hydrolase	-
AOY68_RS00750 (153534)	153534..153845	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY68_RS00755 (153846)	153846..154136	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY68_RS00760 (154741)	154741..154959	+	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY68_RS00765 (155178)	155178..155420	+	243	WP_001087409.1	protein YiiF	-
AOY68_RS00770 (155750)	155750..156679	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY68_RS00775 (156676)	156676..157311	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY68_RS00780 (157308)	157308..158210	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103758 WP_000897305.1 NZ_CP028759:153534-153845 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103758 NZ_CP028759:153534-153845 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103758 WP_000356397.1 NZ_CP028759:153846-154136 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103758 NZ_CP028759:153846-154136 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7