Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1040403..1040944 Replicon chromosome
Accession NZ_CP028758
Organism Escherichia coli strain A16

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag AOY69_RS04835 Protein ID WP_000615980.1
Coordinates 1040403..1040681 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag AOY69_RS04840 Protein ID WP_000729709.1
Coordinates 1040684..1040944 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY69_RS04820 (1037985) 1037985..1038563 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY69_RS04825 (1038769) 1038769..1039536 + 768 WP_000333379.1 class II glutamine amidotransferase -
AOY69_RS04830 (1039507) 1039507..1040247 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY69_RS04835 (1040403) 1040403..1040681 - 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY69_RS04840 (1040684) 1040684..1040944 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY69_RS04845 (1041130) 1041130..1041903 + 774 WP_123007404.1 C40 family peptidase -
AOY69_RS04850 (1042079) 1042079..1042576 + 498 WP_086217930.1 REP-associated tyrosine transposase RayT -
AOY69_RS04855 (1042769) 1042769..1044481 - 1713 Protein_929 flagellar biosynthesis protein FlhA -
AOY69_RS04860 (1044453) 1044453..1045238 + 786 WP_086217936.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1019586..1042576 22990


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T103736 WP_000615980.1 NZ_CP028758:c1040681-1040403 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103736 NZ_CP028758:c1040681-1040403 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT103736 WP_000729709.1 NZ_CP028758:c1040944-1040684 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103736 NZ_CP028758:c1040944-1040684 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References