Detailed information of TA system TA103701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3702497..3703334
• Replicon: chromosome
• Accession: NZ_CP028756
• Organism: Escherichia coli strain A19

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY72_RS17995
• Protein ID: WP_000227784.1
• Coordinates: 3702792..3703334 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY72_RS17990
• Protein ID: WP_001297137.1
• Coordinates: 3702497..3702808 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY72_RS17965 (3697517)	3697517..3698464	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
AOY72_RS17970 (3698486)	3698486..3700477	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY72_RS17975 (3700467)	3700467..3701081	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY72_RS17980 (3701081)	3701081..3701410	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY72_RS17985 (3701422)	3701422..3702312	+	891	WP_000971336.1	heme o synthase	-
AOY72_RS17990 (3702497)	3702497..3702808	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY72_RS17995 (3702792)	3702792..3703334	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY72_RS18000 (3703390)	3703390..3704325	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
AOY72_RS18005 (3704733)	3704733..3706097	+	1365	WP_001000978.1	MFS transporter	-
AOY72_RS18010 (3706225)	3706225..3706716	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY72_RS18015 (3706884)	3706884..3707795	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T103701 WP_000227784.1 NZ_CP028756:3702792-3703334 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T103701 NZ_CP028756:3702792-3703334 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT103701 WP_001297137.1 NZ_CP028756:3702497-3702808 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT103701 NZ_CP028756:3702497-3702808 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3