Detailed information of TA system TA103627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4551137..4551739
• Replicon: chromosome
• Accession: NZ_CP028753
• Organism: Escherichia coli strain A22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AOY75_RS21925
• Protein ID: WP_000897302.1
• Coordinates: 4551428..4551739 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY75_RS21920
• Protein ID: WP_000356397.1
• Coordinates: 4551137..4551427 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY75_RS21895 (4547210)	4547210..4548112	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY75_RS21900 (4548109)	4548109..4548744	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY75_RS21905 (4548741)	4548741..4549670	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY75_RS21910 (4549886)	4549886..4550104	-	219	WP_001314326.1	CopG family transcriptional regulator	-
AOY75_RS21915 (4550500)	4550500..4550778	-	279	WP_001296612.1	hypothetical protein	-
AOY75_RS21920 (4551137)	4551137..4551427	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY75_RS21925 (4551428)	4551428..4551739	-	312	WP_000897302.1	hypothetical protein	Toxin
AOY75_RS21930 (4551968)	4551968..4552876	+	909	WP_001331553.1	alpha/beta hydrolase	-
AOY75_RS21935 (4552940)	4552940..4553881	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY75_RS21940 (4553926)	4553926..4554363	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY75_RS21945 (4554360)	4554360..4555232	-	873	WP_240727533.1	virulence factor BrkB family protein	-
AOY75_RS21950 (4555226)	4555226..4555825	-	600	WP_001314324.1	glucose-1-phosphatase	-
AOY75_RS21955 (4555924)	4555924..4556709	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T103627 WP_000897302.1 NZ_CP028753:c4551739-4551428 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103627 NZ_CP028753:c4551739-4551428 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103627 WP_000356397.1 NZ_CP028753:c4551427-4551137 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103627 NZ_CP028753:c4551427-4551137 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416