Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3871999..3872549 Replicon chromosome
Accession NZ_CP028753
Organism Escherichia coli strain A22

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AOY75_RS18700 Protein ID WP_001160966.1
Coordinates 3871999..3872313 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY75_RS18705 Protein ID WP_000125566.1
Coordinates 3872316..3872549 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY75_RS18675 (3867674) 3867674..3868960 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
AOY75_RS18680 (3868960) 3868960..3869949 + 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY75_RS18685 (3869946) 3869946..3870767 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY75_RS18690 (3870770) 3870770..3871147 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY75_RS18695 (3871154) 3871154..3872002 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY75_RS18700 (3871999) 3871999..3872313 - 315 WP_001160966.1 CcdB family protein Toxin
AOY75_RS18705 (3872316) 3872316..3872549 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY75_RS18710 (3872635) 3872635..3873114 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY75_RS18715 (3873306) 3873306..3875168 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
AOY75_RS18720 (3875161) 3875161..3875691 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY75_RS18725 (3875799) 3875799..3877130 - 1332 WP_001183207.1 MFS transporter -
AOY75_RS18730 (3877189) 3877189..3877476 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T103619 WP_001160966.1 NZ_CP028753:c3872313-3871999 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103619 NZ_CP028753:c3872313-3871999 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103619 WP_000125566.1 NZ_CP028753:c3872549-3872316 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103619 NZ_CP028753:c3872549-3872316 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References