Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3359499..3360117 Replicon chromosome
Accession NZ_CP028753
Organism Escherichia coli strain A22

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY75_RS16150 Protein ID WP_001291435.1
Coordinates 3359899..3360117 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY75_RS16145 Protein ID WP_000344800.1
Coordinates 3359499..3359873 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY75_RS16135 (3354587) 3354587..3355781 + 1195 Protein_3164 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY75_RS16140 (3355804) 3355804..3358954 + 3151 Protein_3165 efflux RND transporter permease AcrB -
AOY75_RS16145 (3359499) 3359499..3359873 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY75_RS16150 (3359899) 3359899..3360117 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY75_RS16155 (3360291) 3360291..3360842 + 552 WP_033549224.1 maltose O-acetyltransferase -
AOY75_RS16160 (3360958) 3360958..3361428 + 471 WP_000136192.1 YlaC family protein -
AOY75_RS16165 (3361592) 3361592..3363142 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY75_RS16170 (3363184) 3363184..3363537 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY75_RS16180 (3363916) 3363916..3364227 + 312 WP_000409908.1 MGMT family protein -
AOY75_RS16185 (3364258) 3364258..3364830 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103617 WP_001291435.1 NZ_CP028753:3359899-3360117 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103617 NZ_CP028753:3359899-3360117 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103617 WP_000344800.1 NZ_CP028753:3359499-3359873 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103617 NZ_CP028753:3359499-3359873 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References