Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2389712..2390274 Replicon chromosome
Accession NZ_CP028753
Organism Escherichia coli strain A22

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AOY75_RS11375 Protein ID WP_000605675.1
Coordinates 2389712..2389990 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY75_RS11380 Protein ID WP_000781370.1
Coordinates 2389990..2390274 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY75_RS11350 (2385298) 2385298..2385729 - 432 WP_000152305.1 peroxiredoxin OsmC -
AOY75_RS11355 (2386074) 2386074..2386289 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY75_RS11360 (2386391) 2386391..2386528 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY75_RS11365 (2386685) 2386685..2388382 + 1698 WP_000433462.1 malate dehydrogenase -
AOY75_RS11370 (2388516) 2388516..2389526 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AOY75_RS11375 (2389712) 2389712..2389990 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY75_RS11380 (2389990) 2389990..2390274 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY75_RS11385 (2390503) 2390503..2391156 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY75_RS11390 (2391149) 2391149..2392033 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY75_RS11395 (2392046) 2392046..2395093 - 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T103616 WP_000605675.1 NZ_CP028753:2389712-2389990 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103616 NZ_CP028753:2389712-2389990 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103616 WP_000781370.1 NZ_CP028753:2389990-2390274 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103616 NZ_CP028753:2389990-2390274 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References