Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 915150..915804 Replicon chromosome
Accession NZ_CP028752
Organism Escherichia coli strain A23

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AOY76_RS04435 Protein ID WP_000244777.1
Coordinates 915397..915804 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY76_RS04430 Protein ID WP_000354046.1
Coordinates 915150..915416 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY76_RS04405 (910319) 910319..911062 + 744 WP_000951948.1 SDR family oxidoreductase -
AOY76_RS04410 (911119) 911119..912552 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
AOY76_RS04415 (912597) 912597..912908 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AOY76_RS04420 (913072) 913072..913731 + 660 WP_000250274.1 hemolysin III family protein -
AOY76_RS04425 (913927) 913927..914907 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY76_RS04430 (915150) 915150..915416 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY76_RS04435 (915397) 915397..915804 + 408 WP_000244777.1 protein YgfX Toxin
AOY76_RS04440 (915844) 915844..916365 - 522 WP_001055874.1 flavodoxin FldB -
AOY76_RS04445 (916477) 916477..917373 + 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
AOY76_RS04450 (917398) 917398..918108 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY76_RS04455 (918114) 918114..919847 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T103585 WP_000244777.1 NZ_CP028752:915397-915804 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103585 NZ_CP028752:915397-915804 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103585 WP_000354046.1 NZ_CP028752:915150-915416 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103585 NZ_CP028752:915150-915416 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References