Detailed information of TA system TA103526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 150123..150725
• Replicon: chromosome
• Accession: NZ_CP028750
• Organism: Escherichia coli strain A25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY78_RS00720
• Protein ID: WP_000897305.1
• Coordinates: 150123..150434 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY78_RS00725
• Protein ID: WP_000356397.1
• Coordinates: 150435..150725 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY78_RS00695 (146037)	146037..146636	+	600	WP_001314324.1	glucose-1-phosphatase	-
AOY78_RS00700 (146630)	146630..147502	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY78_RS00705 (147499)	147499..147936	+	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
AOY78_RS00710 (147981)	147981..148922	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY78_RS00715 (148986)	148986..149894	-	909	WP_001318161.1	alpha/beta hydrolase	-
AOY78_RS00720 (150123)	150123..150434	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY78_RS00725 (150435)	150435..150725	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY78_RS00730 (151328)	151328..151546	+	219	WP_001346587.1	CopG family transcriptional regulator	-
AOY78_RS00735 (151762)	151762..152691	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AOY78_RS00740 (152688)	152688..153323	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY78_RS00745 (153320)	153320..154222	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103526 WP_000897305.1 NZ_CP028750:150123-150434 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103526 NZ_CP028750:150123-150434 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103526 WP_000356397.1 NZ_CP028750:150435-150725 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103526 NZ_CP028750:150435-150725 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7