Detailed information of TA system TA103417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1207870..1208597
• Replicon: chromosome
• Accession: NZ_CP028745
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AOY83_RS05880
• Protein ID: WP_000547563.1
• Coordinates: 1207870..1208181 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY83_RS05885
• Protein ID: WP_000126294.1
• Coordinates: 1208178..1208597 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY83_RS05850 (1203015)	1203015..1204724	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
AOY83_RS05855 (1204734)	1204734..1205276	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AOY83_RS05860 (1205276)	1205276..1206043	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
AOY83_RS05865 (1206040)	1206040..1206450	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
AOY83_RS05870 (1206443)	1206443..1206910	+	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
AOY83_RS05875 (1206953)	1206953..1207708	+	756	WP_240767922.1	hypothetical protein	-
AOY83_RS05880 (1207870)	1207870..1208181	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AOY83_RS05885 (1208178)	1208178..1208597	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AOY83_RS05890 (1208712)	1208712..1210136	-	1425	WP_215260840.1	6-phospho-beta-glucosidase	-
AOY83_RS05895 (1210145)	1210145..1211602	-	1458	WP_057688061.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AOY83_RS05900 (1211862)	1211862..1212872	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
AOY83_RS05905 (1213021)	1213021..1213548	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T103417 WP_000547563.1 NZ_CP028745:1207870-1208181 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T103417 NZ_CP028745:1207870-1208181 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT103417 WP_000126294.1 NZ_CP028745:1208178-1208597 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT103417 NZ_CP028745:1208178-1208597 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCTGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22