Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1203006..1203624 Replicon chromosome
Accession NZ_CP028740
Organism Escherichia coli strain A35

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY88_RS05695 Protein ID WP_001291435.1
Coordinates 1203006..1203224 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY88_RS05700 Protein ID WP_000344800.1
Coordinates 1203250..1203624 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY88_RS05660 (1198296) 1198296..1198868 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY88_RS05665 (1198899) 1198899..1199210 - 312 WP_000409911.1 MGMT family protein -
AOY88_RS05675 (1199589) 1199589..1199942 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY88_RS05680 (1199984) 1199984..1201534 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY88_RS05685 (1201698) 1201698..1202168 - 471 WP_000136192.1 YlaC family protein -
AOY88_RS05690 (1202284) 1202284..1202835 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY88_RS05695 (1203006) 1203006..1203224 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY88_RS05700 (1203250) 1203250..1203624 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY88_RS05705 (1204170) 1204170..1207319 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY88_RS05710 (1207342) 1207342..1208535 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103282 WP_001291435.1 NZ_CP028740:c1203224-1203006 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103282 NZ_CP028740:c1203224-1203006 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103282 WP_000344800.1 NZ_CP028740:c1203624-1203250 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103282 NZ_CP028740:c1203624-1203250 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References