Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2378118..2378644 Replicon chromosome
Accession NZ_CP028738
Organism Escherichia coli strain A38

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AOY91_RS11565 Protein ID WP_000323025.1
Coordinates 2378357..2378644 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AOY91_RS11560 Protein ID WP_000534858.1
Coordinates 2378118..2378357 (+) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY91_RS11525 (2373452) 2373452..2373941 + 490 Protein_2259 class I SAM-dependent methyltransferase -
AOY91_RS11530 (2374419) 2374419..2374840 + 422 Protein_2260 hypothetical protein -
AOY91_RS11535 (2375475) 2375475..2375846 + 372 WP_001406656.1 helix-turn-helix domain-containing protein -
AOY91_RS11540 (2375884) 2375884..2376546 + 663 Protein_2262 ISNCY family transposase -
AOY91_RS11545 (2376667) 2376667..2376816 + 150 WP_011443592.1 protein YdfW -
AOY91_RS11550 (2377253) 2377253..2377585 - 333 WP_001406658.1 FlxA-like family protein -
AOY91_RS11555 (2377788) 2377788..2378093 - 306 WP_001326990.1 protein YdfV -
AOY91_RS11560 (2378118) 2378118..2378357 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AOY91_RS11565 (2378357) 2378357..2378644 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AOY91_RS11570 (2378716) 2378716..2378871 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AOY91_RS11575 (2379088) 2379088..2379339 + 252 WP_000980994.1 protein Rem -
AOY91_RS11580 (2379406) 2379406..2379684 + 279 WP_012304870.1 hypothetical protein -
AOY91_RS11585 (2379686) 2379686..2380735 + 1050 WP_001265198.1 DUF968 domain-containing protein -
AOY91_RS11590 (2380749) 2380749..2381501 + 753 WP_001047135.1 antitermination protein -
AOY91_RS11595 (2381779) 2381779..2381868 - 90 WP_120795389.1 hypothetical protein -
AOY91_RS11600 (2381923) 2381923..2382135 - 213 WP_000087756.1 cold shock-like protein CspF -
AOY91_RS11605 (2382436) 2382436..2382651 + 216 WP_000066484.1 cold shock-like protein CspB -
AOY91_RS11610 (2383015) 2383015..2383185 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
AOY91_RS11615 (2383405) 2383405..2383620 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2360937..2413816 52879


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T103243 WP_000323025.1 NZ_CP028738:2378357-2378644 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T103243 NZ_CP028738:2378357-2378644 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT103243 WP_000534858.1 NZ_CP028738:2378118-2378357 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT103243 NZ_CP028738:2378118-2378357 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References