Detailed information of TA system TA103209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1353605..1354230
• Replicon: chromosome
• Accession: NZ_CP028737
• Organism: Escherichia coli strain A39

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY92_RS06535
• Protein ID: WP_000911330.1
• Coordinates: 1353832..1354230 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY92_RS06530
• Protein ID: WP_000450524.1
• Coordinates: 1353605..1353832 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY92_RS06505 (1349408)	1349408..1349878	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
AOY92_RS06510 (1349878)	1349878..1350450	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AOY92_RS06515 (1350596)	1350596..1351474	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY92_RS06520 (1351491)	1351491..1352525	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AOY92_RS06525 (1352738)	1352738..1353451	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY92_RS06530 (1353605)	1353605..1353832	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY92_RS06535 (1353832)	1353832..1354230	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY92_RS06540 (1354377)	1354377..1355240	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
AOY92_RS06545 (1355255)	1355255..1357270	+	2016	WP_021565358.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY92_RS06550 (1357344)	1357344..1358042	+	699	WP_000679812.1	esterase	-
AOY92_RS06555 (1358123)	1358123..1358323	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T103209 WP_000911330.1 NZ_CP028737:1353832-1354230 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T103209 NZ_CP028737:1353832-1354230 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT103209 WP_000450524.1 NZ_CP028737:1353605-1353832 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT103209 NZ_CP028737:1353605-1353832 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33