103204

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4781967..4782598 Replicon chromosome
Accession NZ_CP028735
Organism Escherichia coli strain A41

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag AOY94_RS23345 Protein ID WP_001259388.1
Coordinates 4782323..4782598 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY94_RS23340 Protein ID WP_000593555.1
Coordinates 4781967..4782326 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY94_RS23310 (4777080) 4777080..4777553 + 474 WP_021554578.1 PTS sugar transporter subunit IIA -
AOY94_RS23315 (4777550) 4777550..4777831 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
AOY94_RS23320 (4777908) 4777908..4779266 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
AOY94_RS23325 (4779259) 4779259..4780767 + 1509 WP_021554579.1 FGGY-family carbohydrate kinase -
AOY94_RS23330 (4780757) 4780757..4781026 + 270 WP_021554580.1 HPr family phosphocarrier protein -
AOY94_RS23335 (4781058) 4781058..4781918 + 861 WP_021554581.1 class II aldolase -
AOY94_RS23340 (4781967) 4781967..4782326 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY94_RS23345 (4782323) 4782323..4782598 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY94_RS23350 (4782671) 4782671..4783795 - 1125 WP_001314210.1 ABC transporter permease -
AOY94_RS23355 (4783795) 4783795..4786530 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
AOY94_RS23360 (4786527) 4786527..4787594 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4776310..4791450 15140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T103204 WP_001259388.1 NZ_CP028735:c4782598-4782323 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T103204 NZ_CP028735:c4782598-4782323 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT103204 WP_000593555.1 NZ_CP028735:c4782326-4781967 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT103204 NZ_CP028735:c4782326-4781967 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCTGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References