Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3917428..3917978 Replicon chromosome
Accession NZ_CP028734
Organism Escherichia coli strain A42

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag AOY95_RS19015 Protein ID WP_001160966.1
Coordinates 3917428..3917742 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AOY95_RS19020 Protein ID WP_000125566.1
Coordinates 3917745..3917978 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY95_RS18990 (3913103) 3913103..3914389 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
AOY95_RS18995 (3914389) 3914389..3915378 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AOY95_RS19000 (3915375) 3915375..3916196 + 822 WP_001523934.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AOY95_RS19005 (3916199) 3916199..3916576 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AOY95_RS19010 (3916583) 3916583..3917431 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AOY95_RS19015 (3917428) 3917428..3917742 - 315 WP_001160966.1 CcdB family protein Toxin
AOY95_RS19020 (3917745) 3917745..3917978 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AOY95_RS19025 (3918064) 3918064..3918543 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
AOY95_RS19030 (3918735) 3918735..3920597 - 1863 WP_001523933.1 glutathione-regulated potassium-efflux system protein KefC -
AOY95_RS19035 (3920590) 3920590..3921120 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AOY95_RS19040 (3921228) 3921228..3922559 - 1332 WP_001523932.1 MFS transporter -
AOY95_RS19045 (3922618) 3922618..3922905 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T103170 WP_001160966.1 NZ_CP028734:c3917742-3917428 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T103170 NZ_CP028734:c3917742-3917428 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT103170 WP_000125566.1 NZ_CP028734:c3917978-3917745 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT103170 NZ_CP028734:c3917978-3917745 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References