Detailed information of TA system TA101741

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3550706..3550931
• Replicon: chromosome
• Accession: NZ_CP028603
• Organism: Escherichia coli strain 144

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: C9E68_RS18725
• Protein ID: WP_000813263.1
• Coordinates: 3550706..3550861 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3550873..3550931 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E68_RS18690	3546160..3546873	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
C9E68_RS18695	3547011..3547207	-	197	Protein_3560	TrmB family transcriptional regulator	-
C9E68_RS18700	3547494..3548312	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
C9E68_RS18705	3548464..3548835	-	372	WP_000090264.1	antitermination protein	-
C9E68_RS18710	3548825..3549196	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
C9E68_RS18715	3549209..3550258	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
C9E68_RS18720	3550260..3550538	-	279	WP_001341388.1	hypothetical protein	-
C9E68_RS18725	3550706..3550861	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3550873..3550931	+	59	-	-	Antitoxin
C9E68_RS18740	3551466..3552239	+	774	WP_000160654.1	alpha/beta hydrolase	-
C9E68_RS18745	3552591..3553004	-	414	WP_001151233.1	DUF977 family protein	-
C9E68_RS18750	3553020..3553790	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
C9E68_RS18755	3553812..3554558	-	747	WP_000788751.1	ATP-binding protein	-
C9E68_RS18760	3554565..3555656	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T101741 WP_000813263.1 NZ_CP028603:c3550861-3550706 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T101741 NZ_CP028603:c3550861-3550706 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101741 NZ_CP028603:3550873-3550931 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin