Detailed information of TA system TA101482

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1867579..1868414
• Replicon: chromosome
• Accession: NZ_CP028578
• Organism: Escherichia coli strain WCHEC005784

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: DBQ99_RS10660
• Protein ID: WP_000854760.1
• Coordinates: 1867579..1867956 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: DBQ99_RS10665
• Protein ID: WP_001360327.1
• Coordinates: 1868046..1868414 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBQ99_RS10620	1863099..1863572	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
DBQ99_RS10625	1863770..1864828	+	1059	WP_001200895.1	FUSC family protein	-
DBQ99_RS10630	1865000..1865329	+	330	WP_000450409.1	DUF496 family protein	-
DBQ99_RS10635	1865430..1865612	-	183	WP_024174802.1	ethanolamine utilization protein	-
DBQ99_RS26390	1866066..1866455	-	390	WP_001445118.1	IS110 family transposase	-
DBQ99_RS10650	1867253..1867366	-	114	WP_032140621.1	DUF957 domain-containing protein	-
DBQ99_RS10655	1867379..1867582	-	204	WP_000761705.1	hypothetical protein	-
DBQ99_RS10660	1867579..1867956	-	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
DBQ99_RS10665	1868046..1868414	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBQ99_RS10670	1868577..1868798	-	222	WP_000692286.1	DUF987 domain-containing protein	-
DBQ99_RS10675	1868861..1869337	-	477	WP_133963824.1	RadC family protein	-
DBQ99_RS10680	1869353..1869826	-	474	WP_000855059.1	antirestriction protein	-
DBQ99_RS10690	1870168..1870986	-	819	WP_060581602.1	DUF945 domain-containing protein	-
DBQ99_RS10700	1871141..1871299	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1861822..1863042	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T101482 WP_000854760.1 NZ_CP028578:c1867956-1867579 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T101482 NZ_CP028578:c1867956-1867579 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT101482 WP_001360327.1 NZ_CP028578:c1868414-1868046 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT101482 NZ_CP028578:c1868414-1868046 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3

Antitoxin

• AlphaFold DB: A0A7U9IW59