Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 8164..8765 Replicon plasmid pCTXM15_005784
Accession NZ_CP028576
Organism Escherichia coli strain WCHEC005784

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag DBQ99_RS00105 Protein ID WP_001216034.1
Coordinates 8164..8544 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DBQ99_RS00110 Protein ID WP_001190712.1
Coordinates 8544..8765 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBQ99_RS00050 3214..3954 - 741 WP_029702141.1 tyrosine-type recombinase/integrase -
DBQ99_RS00055 3994..4691 - 698 Protein_6 IS1 family transposase -
DBQ99_RS00065 4746..5048 + 303 Protein_7 transposase -
DBQ99_RS00070 5304..6179 + 876 WP_000239590.1 class A extended-spectrum beta-lactamase CTX-M-15 -
DBQ99_RS00075 6226..6558 - 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
DBQ99_RS00080 6572..6844 + 273 Protein_10 DUF4158 domain-containing protein -
DBQ99_RS00085 6905..7609 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
DBQ99_RS26315 7674..7952 - 279 Protein_12 pdcB -
DBQ99_RS00100 7980..8159 - 180 WP_001513661.1 hypothetical protein -
DBQ99_RS00105 8164..8544 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DBQ99_RS00110 8544..8765 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DBQ99_RS00115 8948..10504 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
DBQ99_RS00120 10501..11772 + 1272 WP_023142242.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-15 / aac(3)-IId / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) - 1..112422 112422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T101468 WP_001216034.1 NZ_CP028576:c8544-8164 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T101468 NZ_CP028576:c8544-8164 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT101468 WP_001190712.1 NZ_CP028576:c8765-8544 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT101468 NZ_CP028576:c8765-8544 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References