Detailed information of TA system TA101067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3394257..3394984
• Replicon: chromosome
• Accession: NZ_CP028379
• Organism: Escherichia coli O145 str. RM9872

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: I3Y_RS17690
• Protein ID: WP_000547563.1
• Coordinates: 3394673..3394984 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3Y_RS17685
• Protein ID: WP_000126294.1
• Coordinates: 3394257..3394676 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Y_RS17665	3389306..3389833	-	528	WP_001078777.1	electron transport protein HydN	-
I3Y_RS17670	3389982..3390992	-	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
I3Y_RS17675	3391253..3392710	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I3Y_RS17680	3392719..3394143	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
I3Y_RS17685	3394257..3394676	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I3Y_RS17690	3394673..3394984	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
I3Y_RS17695	3395146..3395901	-	756	WP_001400787.1	hypothetical protein	-
I3Y_RS17700	3395944..3396411	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
I3Y_RS17705	3396404..3396814	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
I3Y_RS17710	3396811..3397578	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
I3Y_RS17715	3397578..3398120	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I3Y_RS17720	3398130..3399839	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T101067 WP_000547563.1 NZ_CP028379:c3394984-3394673 [Escherichia coli O145 str. RM9872]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T101067 NZ_CP028379:c3394984-3394673 [Escherichia coli O145 str. RM9872]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT101067 WP_000126294.1 NZ_CP028379:c3394676-3394257 [Escherichia coli O145 str. RM9872]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT101067 NZ_CP028379:c3394676-3394257 [Escherichia coli O145 str. RM9872]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22