Detailed information of TA system TA100980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4518275..4519074
• Replicon: chromosome
• Accession: NZ_CP028320
• Organism: Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: C7X15_RS22305
• Protein ID: WP_000347252.1
• Coordinates: 4518610..4519074 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: C7X15_RS22300
• Protein ID: WP_001296435.1
• Coordinates: 4518275..4518610 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7X15_RS22285	4514060..4514830	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
C7X15_RS22290	4514846..4516180	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
C7X15_RS22295	4516555..4518126	+	1572	WP_001273940.1	galactarate dehydratase	-
C7X15_RS22300	4518275..4518610	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C7X15_RS22305	4518610..4519074	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C7X15_RS22310	4519129..4519938	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C7X15_RS22315	4520187..4521467	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C7X15_RS22320	4521490..4521963	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C7X15_RS22325	4521974..4522753	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C7X15_RS22330	4522743..4523621	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C7X15_RS22335	4523639..4524073	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T100980 WP_000347252.1 NZ_CP028320:4518610-4519074 [Escherichia coli O18:H1]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T100980 NZ_CP028320:4518610-4519074 [Escherichia coli O18:H1]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT100980 WP_001296435.1 NZ_CP028320:4518275-4518610 [Escherichia coli O18:H1]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT100980 NZ_CP028320:4518275-4518610 [Escherichia coli O18:H1]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5