Detailed information of TA system TA100678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 49363..49947
• Replicon: plasmid pAbNCIMB8209_134
• Accession: NZ_CP028139
• Organism: Acinetobacter baumannii strain NCIMB 8209

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: C4X49_RS18725
• Protein ID: WP_000286963.1
• Coordinates: 49363..49665 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C4X49_RS18730
• Protein ID: WP_000985610.1
• Coordinates: 49666..49947 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4X49_RS18700	45366..46046	-	681	WP_004832927.1	substrate binding domain-containing protein	-
C4X49_RS18705	46085..47293	-	1209	WP_004832869.1	IS256-like element ISAba26 family transposase	-
C4X49_RS18710	47530..47754	+	225	WP_125510319.1	hypothetical protein	-
C4X49_RS18715	47788..48150	+	363	Protein_50	plasmid replication DNA-binding protein	-
C4X49_RS18720	48350..49171	-	822	WP_017725646.1	GIY-YIG nuclease family protein	-
C4X49_RS18725	49363..49665	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4X49_RS18730	49666..49947	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
C4X49_RS18735	50151..51023	+	873	WP_006581603.1	restriction endonuclease	-
C4X49_RS18740	51644..52000	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4X49_RS18745	51993..52295	+	303	WP_001140619.1	XRE family transcriptional regulator	-
C4X49_RS18750	52288..52497	+	210	WP_000069471.1	hypothetical protein	-
C4X49_RS18755	52607..52825	-	219	WP_029747731.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133709	133709
	-	flank	IS/Tn	-	-	46085..47293	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T100678 WP_000286963.1 NZ_CP028139:49363-49665 [Acinetobacter baumannii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T100678 NZ_CP028139:49363-49665 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT100678 WP_000985610.1 NZ_CP028139:49666-49947 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT100678 NZ_CP028139:49666-49947 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCGATTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2