Detailed information of TA system TA100499

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1326051..1326422
• Replicon: chromosome
• Accession: NZ_CP028112
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: I3Q_RS06905
• Protein ID: WP_042853000.1
• Coordinates: 1326228..1326422 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1326051..1326229 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS06865	1321493..1322149	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
I3Q_RS06870	1322251..1322481	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
I3Q_RS06875	1322620..1322994	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
I3Q_RS06880	1322998..1323870	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
I3Q_RS06885	1323883..1324224	+	342	WP_000976492.1	YebY family protein	-
I3Q_RS06890	1324617..1325693	-	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
I3Q_RS06895	1325659..1325940	-	282	WP_001443927.1	excisionase	-
I3Q_RS06900	1326047..1326235	-	189	WP_001356607.1	DUF1187 family protein	-
-	1326051..1326229	+	179	NuclAT_1	-	Antitoxin
-	1326051..1326229	+	179	NuclAT_1	-	Antitoxin
-	1326051..1326229	+	179	NuclAT_1	-	Antitoxin
-	1326051..1326229	+	179	NuclAT_1	-	Antitoxin
I3Q_RS06905	1326228..1326422	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I3Q_RS06910	1326479..1327288	-	810	WP_000166317.1	recombination protein RecT	-
I3Q_RS06915	1327281..1329932	-	2652	WP_000105102.1	exodeoxyribonuclease VIII	-
I3Q_RS06920	1330031..1330306	-	276	WP_001307773.1	hypothetical protein	-
I3Q_RS06925	1330380..1330556	-	177	WP_000245534.1	hypothetical protein	-
I3Q_RS06930	1330550..1330771	-	222	WP_000560218.1	killing protein KilR	-
I3Q_RS06935	1331192..1331344	-	153	WP_000379547.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	1318750..1395539	76789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T100499 WP_042853000.1 NZ_CP028112:c1326422-1326228 [Escherichia coli O103 str. RM8385]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T100499 NZ_CP028112:c1326422-1326228 [Escherichia coli O103 str. RM8385]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT100499 NZ_CP028112:1326051-1326229 [Escherichia coli O103 str. RM8385]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin