Detailed information of TA system TA100475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3806692..3807317
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I3S_RS20115
• Protein ID: WP_000911330.1
• Coordinates: 3806919..3807317 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I3S_RS20110
• Protein ID: WP_000450524.1
• Coordinates: 3806692..3806919 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS20085	3802495..3802965	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
I3S_RS20090	3802965..3803537	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
I3S_RS20095	3803683..3804561	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I3S_RS20100	3804578..3805612	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I3S_RS20105	3805825..3806538	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I3S_RS20110	3806692..3806919	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I3S_RS20115	3806919..3807317	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I3S_RS20120	3807464..3808327	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
I3S_RS20125	3808342..3810357	+	2016	WP_000829325.1	tRNA cytosine(34) acetyltransferase TmcA	-
I3S_RS20130	3810431..3811128	+	698	Protein_3671	esterase	-
I3S_RS20135	3811238..3811438	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T100475 WP_000911330.1 NZ_CP028110:3806919-3807317 [Escherichia coli O121 str. RM8352]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T100475 NZ_CP028110:3806919-3807317 [Escherichia coli O121 str. RM8352]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT100475 WP_000450524.1 NZ_CP028110:3806692-3806919 [Escherichia coli O121 str. RM8352]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT100475 NZ_CP028110:3806692-3806919 [Escherichia coli O121 str. RM8352]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33